Map of DnaC localization in the presence or absence of PcrA (PcrA depletion)

The replicative helicase, DnaC, was immunoprecipitated in cell extract prior to, or following conditional depletion of PcrA The differential DnaC association at each genomic location was determined before/after PcrA depletion

在针对PcrA的条件性耗竭（conditional depletion）前后，我们均在细胞提取物中对复制解旋酶（replicative helicase）DnaC开展了免疫沉淀（immunoprecipitation）实验；并测定了PcrA条件性耗竭前后，DnaC在各基因组位点的结合差异。