Raw and normalized inhibitory activities for all nsp1 mutants.

The inhibitory functions for all nsp1 mutants are shown in this table expressed as averaged percent compared to nsp1-wt (set to 100 percent). Percent inhibitions were also normalized to expression levels. No values are reported for mutants nsp1-m8, -m25, and -m26 as they had no significant inhibitory activities and were expressed at a level too low to be measured. After expression normalization, many nsp1 mutants exhibited increased inhibitory abilities compared to nsp1-wt; for example, nsp1-m19 exhibits nearly four-fold more inhibitory functions over nsp1-wt. Even after normalization, nsp1-m16 continues to show a loss of inhibition of host IFN- and virus-dependent signaling while maintaining strong inhibition of host gene expression. (*indicates significant as compared to empty vector; #indicates significant as compared to nsp1-wt; significant is defined as a t-Test P-value ≤ 0.05).

本表格展示了所有nsp1突变体的抑制功能，以相对于野生型nsp1（设为100%）的平均百分比形式呈现。各抑制百分比已根据表达水平完成标准化处理。突变体nsp1-m8、nsp1-m25及nsp1-m26未报告相关数值，因其无显著抑制活性，且表达水平过低无法被检测。经表达标准化后，相较于野生型nsp1，多数nsp1突变体的抑制能力有所提升；例如nsp1-m19的抑制功能几乎为野生型nsp1的4倍。即便经过标准化处理，nsp1-m16仍表现出对宿主干扰素（Interferon，IFN）及病毒依赖型信号通路的抑制能力丧失，但同时对宿主基因表达仍保有强效抑制作用。（*表示与空载体对照组相比具有显著性差异；#表示与野生型nsp1相比具有显著性差异；显著性定义为t检验P值≤0.05）