Expression data from Bone marrow and Fetal liver Lineage-c-Kit+Sca-1+ (LSK) cells. Mus musculus

Loss of polycomb-group gene Ezh2 causes activation of fetal gene signature in adult mouse bone marrow (BM) hematopoietic stem and progenitor cells (HSPCs). Ezh2 directly represses fetal-specific let-7 target genes, including Lin28, thereby cooperates with let-7 microRNAs in silencing fetal gene signature in BM HSPCs. Overall design: We purified Lineage-Sca-1+c-Kit+ (LSK) HSPCs from E14.5 FL and adult BM and subjected them to microarray analysis.

多梳家族基因Ezh2（polycomb-group gene Ezh2）的缺失会导致成年小鼠骨髓（bone marrow, BM）造血干祖细胞（hematopoietic stem and progenitor cells, HSPCs）激活胎儿基因特征（fetal gene signature）。Ezh2可直接抑制胎儿特异性let-7靶基因，包括Lin28，从而与let-7微RNA（let-7 microRNAs）协同沉默骨髓造血干祖细胞中的胎儿基因特征。整体实验设计：我们从胚胎第14.5天胎肝（E14.5 fetal liver, FL）与成年小鼠骨髓中纯化得到谱系-Sca-1+c-Kit+（LSK）造血干祖细胞，并对其进行微阵列分析（microarray analysis）。