Enterococcus phage phiEf11 genome sequencing project. Enterococcus phage phiEf11 strain:TUSoD11

Phage phiEf11 was induced from lysogenic E. faecalis strain TUSoD11, and purified as previously described (Stevens et al 2009). Stevens R.H., Porras O.D., and Delisle A.L. 2009. Bacteriophages induced from lysogenic root canal isolates of Enterococcus faecalis. Oral Microbiol. Immunol. 24: 278-284. Recently, we isolated temperate bacteriophages that were induced from E. faecalis strains recovered from the infected root canals of teeth that had previously undergone endodontic treatment (Stevens et al 2009). One of the isolates, designated phage phiEf11, was characterized as a Siphovirdae morphotype, with a spherical head and a long noncontactile tail. Analysis of NdeI and NsiI restriction fragments indicated a DNA length of approximately 41 kbp. To further our understanding of this virus, and explore its potential for either contributing to, or mitigating against, the pathogenicity of its host cell, we undertook the sequencing and functional analysis of its complete genome.

噬菌体phiEf11从溶源性粪肠球菌（Enterococcus faecalis）菌株TUSoD11中诱导获得，并按照此前报道的方法完成纯化（Stevens等，2009）。Stevens R.H.、Porras O.D.与Delisle A.L.于2009年发表研究《从粪肠球菌溶源性根管分离株中诱导得到的噬菌体》，刊载于《Oral Microbiol. Immunol.》24卷：278-284页。近期，我们从曾接受牙髓治疗的患牙感染根管内分离得到的粪肠球菌菌株中，诱导获得温和噬菌体（temperate bacteriophages），该类群此前已由Stevens等2009年的研究报道。其中一株被命名为phiEf11的分离株，经鉴定属于Siphovirdae形态型，具有球形头部与长非收缩尾。对NdeI和NsiI限制性酶切片段的分析结果显示，其基因组DNA长度约为41 kbp。为进一步解析该噬菌体的生物学特性，并探究其对宿主细胞致病性的促进或缓解潜力，我们对其完整基因组开展了测序与功能分析。