Genome-wide transcriptome analysis of Esrrb WT and conditional Esrrb KO mouse embryonic stem cells. Genome-wide transcriptome analysis of Esrrb WT and conditional Esrrb KO mouse embryonic stem cells

We report the application of genome-wide RNA-sequencing analysis for Esrrb WT and conditional Esrrb KO mES cells after differentation for 48 hours. Global transcriptome profiling revealed impaired induction of formative genes in conditional KO cells compared to WT cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for E14 and Esrrb KO cells stably transfected either with an inducible Empty vector or with an inducible vector expressing Esrrb. Cells were maintained with DOX and 2iL and subsequently differentiated for 48 hours in absence of 2iL and DOX.

本研究报道了针对经48小时诱导分化的Esrrb野生型（Esrrb WT）与条件性Esrrb敲除（Esrrb KO）小鼠胚胎干细胞（mouse embryonic stem cells, mES cells）开展的全基因组RNA测序（genome-wide RNA-sequencing）分析应用。全转录组分析（global transcriptome profiling）结果显示，相较于野生型细胞，条件性敲除细胞内的成型性基因诱导过程存在功能受损情况。总体实验设计：对分别稳定转染诱导型空载体（inducible Empty vector）与诱导型Esrrb表达载体（inducible vector expressing Esrrb）的E14细胞系及Esrrb KO细胞的RNA测序数据开展比较基因表达谱分析。细胞在添加多西环素（doxycycline, DOX）与2iL的培养基中维持培养，随后在不含2iL与多西环素的培养基中诱导分化48小时。