The human β-globin enhancer LCR HS2 plays a role in forming a TAD by activating chromatin structure at neighboring CTCF sites. The human β-globin enhancer LCR HS2 plays a role in forming a TAD by activating chromatin structure at neighboring CTCF sites

We propose that the HS2 has a role in forming a β-globin TAD by activating neighboring CTCF sites and the role is beyond typical enhancer activity. Overall design: H3K27ac, H3K27me3 ChIP-seq was performed in control and LCR HS2 deletion cells.

我们提出，HS2可通过激活邻近的CCCTC结合因子（CCCTC-binding factor，CTCF）结合位点，参与β-珠蛋白拓扑相关结构域（Topologically Associating Domain，TAD）的构建，且该功能超越了典型增强子的活性范畴。实验设计：在对照组及基因座控制区（Locus Control Region，LCR）HS2缺失细胞中，分别开展组蛋白H3赖氨酸27乙酰化（histone H3 lysine 27 acetylation，H3K27ac）、组蛋白H3赖氨酸27三甲基化（histone H3 lysine 27 trimethylation，H3K27me3）的染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing，ChIP-seq）实验。