U2OS osteosarcoma cells LC-MSMS

In order to identify the targets of miR-193a-5p in osteosarcoma U2OS cell line, we used a lentivirus-mediated expression system to overexpressing miR-193a precusor, miR-193a-5p target sequence and non-target sequence, respectively, in osteosarcoma cell line U2OS. A tandem mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-193a-5p-regulated proteins. order to identify the targets of miR-193a-5p, we used a lentivirus-mediated expression system to overexpressing miR-193a precusor, miR-193a-5p target sequence and non-target sequence, respectively, in osteosarcoma cell line U2OS. A tandem mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-193a-5p-regulated proteins.

为鉴定骨肉瘤U2OS细胞系中微小RNA-193a-5p（miR-193a-5p）的靶标，我们采用慢病毒介导的表达系统，分别在骨肉瘤U2OS细胞系中过表达miR-193a前体、miR-193a-5p靶序列及非靶序列。随后运用基于串联质量标签（Tandem Mass Tag, TMT）的定量蛋白质组学策略，以鉴定miR-193a-5p调控的蛋白质全局表达谱。