Anti-IgE Effect of Naturally Occurring Arctigenin on Food Allergy Association with a Distinct Transcriptome Profile

Purpose: To identify IgE inhibitory bioactive compound from A.Lappa for targeted therapy on peanut specific IgE production and hypersensitivity reactions, and to investigate its underlying mechanisms. The goals of this study were to compare the transcriptome map (RNA-seq) and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) before and after PMBC in patients with food allergy, and to evaluate the most critical genes and signaling pathways which inhibit IgE production by arctigenin. IgE inhibitory effects on human B cells (U266 human myeloma cells) and peripheral blood mononuclear cells (PBMCs) from food allergic patients, and in peanut sensitized and challenged murine model were assessed. And genes involved in IgE inhibition were studied with transcriptome sequencing. Examination of 3 set of transcriptome profiles of PBMCs (control, IL-4/anti-CD40 stimulation, IL-4/anti-CD40 stimulation+arctigenin).

研究目的：从牛蒡（A.Lappa）中筛选可抑制免疫球蛋白E（Immunoglobulin E，IgE）产生的生物活性成分，用于针对花生特异性IgE产生与超敏反应的靶向治疗，并探究其潜在作用机制。本研究的目标为：对比食物过敏患者外周血单个核细胞（PBMCs）处理前后的转录组图谱（RNA测序，RNA-seq）与定量逆转录聚合酶链反应（qRT-PCR）结果，评估牛蒡子苷元（arctigenin）抑制IgE产生过程中发挥关键调控作用的基因与信号通路。本研究同时评估了牛蒡子苷元对人类B细胞（U266人骨髓瘤细胞）、食物过敏患者外周血单个核细胞（PBMCs）以及花生致敏激发小鼠模型的IgE抑制活性，并通过转录组测序分析参与IgE抑制过程的相关基因。本次实验共设置3组PBMC转录组谱：对照组、白细胞介素4/抗CD40刺激（IL-4/anti-CD40 stimulation）组、白细胞介素4/抗CD40刺激+牛蒡子苷元组。