RNA-Seq of Saccharomyces cerevisiae cells carrying different YRR1 alleles in response to 4-nitroquinoline-N-oxide (4NQO) and to glycerol as the sole carbon source

In this study, we constructed three isogenic strains of S96 yrr1Δ background (its native YRR1 gene was knocked out) carrying three different YRR1 alleles, YRR1_S96, YRR1_YJM789 and YRR1_S96-I775E, respectively. We then conducted RNA deep sequencing (RNA-Seq) on the three strains grown in Yeast Peptone Dextrose medium (YPD), YPD + 4NQO and Yeast Peptone glycerol medium (YPglycerol). RNA-Seq was performed in biological duplicates on S96 cells carrying three different YRR1 alleles grown in YPD, YPD + 4NQO, YPglycerol

本研究构建了三株处于S96 yrr1Δ背景（即其原生YRR1基因已被敲除）的同基因菌株（isogenic strains），分别携带三种不同的YRR1等位基因：YRR1_S96、YRR1_YJM789与YRR1_S96-I775E。随后，我们对分别在酵母蛋白胨葡萄糖培养基（Yeast Peptone Dextrose medium, YPD）、添加4-硝基喹啉氮氧化物（4NQO）的YPD培养基以及酵母蛋白胨甘油培养基（Yeast Peptone glycerol medium, YPglycerol）中培养的上述三株菌株开展了RNA深度测序（RNA deep sequencing, RNA-Seq）。针对在YPD、添加4NQO的YPD以及YPglycerol培养基中培养的携带三种不同YRR1等位基因的S96细胞，我们同样进行了生物学重复（biological duplicates）的RNA-Seq检测。