The effect on cancer cells of the glycodendrimers believed to decrease tumor growth, prolong survival time, and enhance immune response. Homo sapiens

The potential therapeutic relevance of synthetic glycodendrimers was shown on animal subjects bearing colorectal carcinoma and melanoma. The application of glycodendrimers led to decreased tumor growth and prolonged survival time of the animals, accompanied by an enhancement of immune response. Comparison of mass spectra and biochemical analyses have indicated that the uptake of glycodendrimers by target cells resulted in changes of surface glycosylation. Moreover our research shows changes in membrane constitution. Recently our lab found changes in proliferation potential and expression of GnT-III and GnT-V in cancer cells in contrast to the peripheral blood mononuclear cells from healthy donors. As there are a sizable amount of enzymes involved in glycan metabolism and signaling pathways, the glyco-chip would be a very effective tool to determine which genes are involved in metabolism of these glycodendrimers. Our lab would like to develop expression profiles of cancer cells treated by glycodendrimers and a corresponding untreated control. Overall design: For chip analyses we have chosen cell lines derived from chronic myeloid leukemia (K562), multiple myeloma (U266) and colon carcinoma (HT-29). There are untreated control samples and post glycodendrimers treatment samples from each cell line. All samples were produced and developed in triplicate for a total of 18 samples. The RNA was labeled and hybridized onto the GLYCOv3 array. .

本研究证实了合成糖树状大分子（synthetic glycodendrimers）在携带有结直肠癌与黑色素瘤的动物模型中的潜在治疗价值。实验结果表明，糖树状大分子可抑制肿瘤生长、延长动物存活时间，并增强机体免疫应答。质谱比对与生化分析显示，靶细胞摄取糖树状大分子后会引发表面糖基化模式改变；此外本研究还发现细胞膜组成发生变化。近期本实验室发现，与健康供者的外周血单个核细胞相比，癌细胞的增殖潜能以及GnT-III、GnT-V的表达均出现显著改变。由于糖代谢与信号通路涉及大量酶类，糖芯片（glyco-chip）可作为高效工具，用于筛选参与这类糖树状大分子代谢的相关基因。本实验室拟构建经糖树状大分子处理的癌细胞与对应未处理对照组的基因表达谱。实验设计概述：芯片分析选用了慢性髓系白血病（K562）、多发性骨髓瘤（U266）以及结肠癌（HT-29）来源的细胞系；每种细胞系均设置未处理对照样本与糖树状大分子处理后样本；所有样本均设置3次生物学重复，总计18份样本。对提取的RNA进行标记后，将其与GLYCOv3芯片进行杂交。