MAGs-dbCAN_KoFamScan_annotation.txt

Metagenome-assembled genomes (MAGs) were recovered from individual and co-assemblies (MEGAHIT v1.2.9) using MaxBin2 v2.2.7 and MetaBAT v2.12.1 (contig > 2000 bp) followed by dereplication with dRep v3.2.2 (algorithm gANi, P_ani: 0.90, S_ani: 0.97) resulting in 144 MAGs.This file includes the metagenomic annotations with dbCAN (dbCAN-HMMdb-V12 (2023-08-02)) and KoFamScan including expression data for the MAGs from the DENITRO-FDB project.

本研究从单样本组装与共组装结果（采用MEGAHIT v1.2.9完成组装）中获取宏基因组组装基因组（Metagenome-assembled genomes, MAGs），使用MaxBin2 v2.2.7与MetaBAT v2.12.1工具进行提取，筛选条件为contig长度大于2000 bp；随后使用dRep v3.2.2进行去冗余处理，去冗余算法为gANi，P_ani阈值设为0.90，S_ani阈值设为0.97，最终获得144个MAGs。本文件包含基于dbCAN数据库（dbCAN-HMMdb-V12，2023-08-02）与KoFamScan完成的宏基因组注释信息，以及来自DENITRO-FDB项目的MAGs表达数据。