VEPH1 expression in ovarian cancer cell lines and its relationship to TGF-B signaling

The current study examines the functional role of VEPH1, a PH domain containing protein and the human ortholog of Drosophila melted, in ovarian cancer cell lines. Elevated VEPH1 is associated with FOXO and Hippo signaling and was found to suppress TGF-ß induced target genes. SKOV3 cell lines were established as either CdCl2 inducible Flag-tagged VEPH1 expression or mock-transfected. Gene expression profiles were then generated both with and without the presence of TGF-ß to induce TGF-ß signaling. 3 replicates per treatment group were used.

本研究探究了卵巢癌细胞系中VEPH1的功能作用：VEPH1是一类含PH结构域（PH domain）的蛋白，同时为果蝇melted的人类同源蛋白。高表达的VEPH1与FOXO信号通路、Hippo信号通路存在密切关联，且被证实可抑制转化生长因子-β（TGF-β）诱导的靶基因表达。研究人员构建了两种SKOV3细胞系：一类为经氯化镉（CdCl₂）诱导的Flag标签化VEPH1过表达细胞系，另一类为空白转染对照细胞系。随后，在分别添加与不添加转化生长因子-β以激活TGF-β信号通路的条件下，获取了细胞的基因表达谱。每个处理组均设置3次生物学重复。