Antibody repertoire RNA-seq of BALB/c and C57BL/6 baseline mice (blood and spleen). Antibody repertoire RNA-seq of BALB/c and C57BL/6 baseline mice (blood and spleen)

B cell receptor repertoire is with huge diversity among mouse individuals that hamper the search for true signals caused by targeted experimental factors. The data was sequenced for the measurement of the spectrum similarity among baseline mice. It aimed to compare and assess the convergence of the repertoire in two mouse strains (BALB/c, n=8 and C57BL/6, n=5) and two kinds of sample type (PBMC form blood and splenocyte form spleen). Among them, each sample from 3 BALB/c mice were divided into two and sequenced respectively. 5' RACE method and a set of mixed specific primers for IgA/G/M isotypes were applied to amplify the targeted region of IgH.

小鼠个体间的B细胞受体库（B cell receptor repertoire）存在极高多样性，这极大阻碍了针对靶向实验因素诱导的真实信号的挖掘。本数据集通过测序获得，用于测定基线状态小鼠的受体谱相似性。研究旨在比较并评估两种小鼠品系（BALB/c，n=8；C57BL/6，n=5）与两种样本类型（血液来源的外周血单个核细胞（peripheral blood mononuclear cell, PBMC）、脾脏来源的脾细胞）的受体库趋同性。其中，3只BALB/c小鼠的每份样本均被均分为两份并分别进行测序。本研究采用5' 末端快速扩增（5' rapid amplification of cDNA ends, 5' RACE）技术，以及针对IgA、IgG、IgM同种型的混合特异性引物组合，对免疫球蛋白重链（IgH）的目标区域进行扩增。