Endophytic bacterial communities in peels and pulp of five root vegetables. Microbes in root vegetables

Plants contain endophytic bacteria, whose communities both influence plant growth and can be an important source of probiotics. Here we used deep sequencing of a 16S rRNA gene fragment and bacterial cultivation to independently characterize the microbiomes of five plant species from divergent taxonomic orders - potato (Solanum tuberosum), carrot (Daucus sativus), beet (Beta vulgaris), neep (Brassica napus spp. napobrassica), and topinambur (Helianthus tuberosus). We found that both species richness and diversity tend to be higher in the peel, where Alphaproteobacteria and Actinobacteria dominate, while Gammaproteobacteria and Firmicutes dominate in the pulp.

植物体内定殖有内生细菌（endophytic bacteria），其菌群既会影响植物生长，同时也可作为益生菌的重要来源。本研究采用16S rRNA基因片段深度测序与细菌培养两种方法，独立解析了来自不同分类学目的5种植物的微生物组，供试植物包括马铃薯（Solanum tuberosum）、胡萝卜（Daucus sativus）、甜菜（Beta vulgaris）、芜菁甘蓝（Brassica napus spp. napobrassica）以及菊芋（Helianthus tuberosus）。研究发现，果皮中的物种丰富度与多样性均趋于更高水平，其中α-变形菌门（Alphaproteobacteria）和放线菌门（Actinobacteria）占据优势，而果肉中则以γ-变形菌门（Gammaproteobacteria）和厚壁菌门（Firmicutes）为主导类群。