DataSheet2_Immunosuppressive Isopimarane Diterpenes From Cultures of the Endophytic Fungus Ilyonectria robusta.docx

Five new isopimarane diterpenes, robustaditerpene A-E (1–5), which include 19-nor-isopimarane skeleton and isopimarane skeleton, were isolated from the liquid fermentation of the endophytic fungus Ilyonectria robusta collected from Bletilla striata. The structure elucidation and relative configuration assignments of all compounds were accomplished by interpretation of NMR and HRESIMS spectrometric analyses and 13C NMR calculation. And the absolute configuration of 1-5 were identified by single-crystal X-ray diffraction and ECD calculation. Compound 3 inhibited lipopolysaccharide-induced B lymphocytes cell proliferation with an IC50 value at 17.42 ± 1.57 μM while compound 5 inhibited concanavalin A-induced T lymphocytes cell proliferation with an IC50 value at 75.22 ± 6.10 μM. These data suggested that compounds 3 and 5 may possess potential immunosuppressive prospect.

从采集自白及（Bletilla striata）的内生真菌粗壮伊莱霉（Ilyonectria robusta）的液体发酵物中，分离得到5个新的异海松烷（isopimarane）型二萜类化合物，命名为robustaditerpene A-E（1–5），其骨架涵盖19-去甲异海松烷（19-nor-isopimarane）与异海松烷两种类型。所有化合物的结构解析及相对构型确定，均通过核磁共振波谱（NMR）、高分辨电喷雾电离质谱（HRESIMS）数据分析及碳-13核磁共振（13C NMR）计算完成。化合物1–5的绝对构型则通过单晶X射线衍射（single-crystal X-ray diffraction）与电子圆二色谱（ECD）计算得以确认。化合物3可抑制脂多糖（lipopolysaccharide）诱导的B淋巴细胞增殖，其半数抑制浓度（IC50）为17.42 ± 1.57 μM；化合物5则可抑制刀豆蛋白A（concanavalin A）诱导的T淋巴细胞增殖，其IC50值为75.22 ± 6.10 μM。上述实验数据表明，化合物3与5具备潜在的免疫抑制应用前景。