RNA sequencing (RNA-seq) of ANKRD6 in MKN45 gastric cancer cells by shRNA. RNA sequencing (RNA-seq) of ANKRD6 in MKN45 gastric cancer cells by shRNA

Metastasis and recurrence are the major causes of poor outcome in gastric cancer (GC). We showed that ANKRD6 expression is associated with a poorer prognosis in 10 independent GC cohorts and with metastatic states and processes. Here, by transcriptomic profiling we have characterized the effect of ANKRD6 silencing in vitro and found that cells with ANKRD6 downregulation display an impairment in key metastatic processes such as the Epithelial-mesenchymal transition. Overall design: The experiment was performed in MKN45 cells expressing shANKRD6 and the control plasmids after lentiviral infection on four independent replicates. After QC, one pair of samples (inf III) was dropped off from the analysis.

转移与复发是导致胃癌（GC）患者预后不良的主要诱因。本研究证实，ANKRD6的表达水平与10个独立胃癌队列的不良预后以及肿瘤转移状态和转移进程密切相关。
本研究通过转录组分析（transcriptomic profiling）体外阐明了ANKRD6基因沉默的生物学效应，发现ANKRD6表达下调的细胞在上皮间质转化（Epithelial-mesenchymal transition）这类关键转移进程中出现功能受损。
实验设计概述：本实验以慢病毒感染后的MKN45细胞为模型，构建了表达shANKRD6的实验组与空载质粒对照组，并设置4次独立生物学重复。质量控制（QC）完成后，1对样本（inf III）被排除出后续分析。