The transcriptomic changes affected by BDF2 and SIR2 under salt stress. Saccharomyces cerevisiae

The strain bdf1∆bdf2∆[BDF2 L][SIR2 H] improved salt resistance of bdf1∆. To gain further insight into the mechanism of BDF1 in suppressing bdf1∆ salt sensitivity, DNA microarray analysis was performed to determine the reason for the salt sensitivity of bdf1∆ cells and the process of how coexpression of SIR2 and BDF2 improves salt resistance. Transcriptomic analysis under salt treatment (0.6 mol.L-1 NaCl for 45 min) was performed using three different strains: bdf1∆bdf2∆[BDF2 L][SIR2 H], bdf1∆bdf2∆[BDF2 L][pYX242] and bdf1∆[pRS316][pYX242]. The transcription of 3244 genes were significantly changed( > 2-fold) in bdf1∆bdf2∆[BDF2 L][SIR2 H] compared with bdf1∆[pRS316][pYX242] upon NaCl stress (0.6 mol.L-1 NaCl for 45 min). Only 281 genes were significantly changed ( > 2-fold) in bdf1∆bdf2∆[BDF2 L][pYX242] compared with bdf1∆[pRS316][pYX242] upon NaCl stress (0.6 mol∙L-1 NaCl for 45 min). BF2:bdf1∆[pRS316][pYX242]. BF2B: bdf1∆bdf2∆[BDF2 L][pYX242]. BF2S:bdf1∆bdf2∆[BDF2 L][SIR2 H]. Overall design: BF2B vs BF2 under salt treatment (0.6 mol.L-1 NaCl for 45 min); BF2S vs BF2 under salt treatment (0.6 mol.L-1 NaCl for 45 min).

菌株bdf1∆bdf2∆[BDF2 L][SIR2 H]可改善bdf1∆的耐盐性。为进一步阐明BDF1抑制bdf1∆盐敏感性的分子机制，本研究通过DNA微阵列（DNA microarray）分析，探究bdf1∆细胞盐敏感性的成因，以及SIR2与BDF2共表达改善耐盐性的具体过程。本研究针对三种不同菌株开展盐胁迫处理（0.6 mol·L⁻¹氯化钠处理45分钟）下的转录组分析，所用菌株分别为bdf1∆bdf2∆[BDF2 L][SIR2 H]、bdf1∆bdf2∆[BDF2 L][pYX242]以及bdf1∆[pRS316][pYX242]。在氯化钠胁迫（0.6 mol·L⁻¹氯化钠处理45分钟）条件下，相较于bdf1∆[pRS316][pYX242]，bdf1∆bdf2∆[BDF2 L][SIR2 H]中共有3244个基因的转录水平发生显著变化（变化倍数>2倍）。同样条件下，相较于bdf1∆[pRS316][pYX242]，bdf1∆bdf2∆[BDF2 L][pYX242]中仅有281个基因的转录水平发生显著变化（变化倍数>2倍）。BF2指代bdf1∆[pRS316][pYX242]；BF2B指代bdf1∆bdf2∆[BDF2 L][pYX242]；BF2S指代bdf1∆bdf2∆[BDF2 L][SIR2 H]。实验整体设计：分别在氯化钠胁迫（0.6 mol·L⁻¹氯化钠处理45分钟）条件下开展BF2B与BF2、BF2S与BF2的对比分析。