Next Generation Sequencing timecourse of murine hepatocytes with or without paracetamol treatment

Untreated mice or mice treated with paracetamol, in the presence or absence of TSA, were culled after 14 hrs or 24 hrs of treatment, and RNAseq was performed. Method: RNA was extracted, in triplicate, then sequenced with an Illumina NextSeq 500 sequencer. Sequence reads passing the quality control filters were aligned using Tophat2 and then analysed with Cufflinks. Overall design: Quadruplicate polyA mRNA profiles from mouse hepatocytes were generated following various drug treatment protocols, using an Illumina NextSeq 500 sequencer.

未经处理的小鼠，或经对乙酰氨基酚（paracetamol）处理且存在或缺失曲古抑菌素A（TSA）的小鼠，在给药处理14小时或24小时后被处死，并进行RNA测序（RNAseq）。
实验方法：将RNA进行一式三份提取，随后使用Illumina NextSeq 500测序仪完成测序。通过质量控制过滤的序列读段，采用Tophat2进行序列比对，再借助Cufflinks完成数据分析。
整体实验设计：使用Illumina NextSeq 500测序仪，针对不同药物处理方案下的小鼠肝细胞，构建了四重重复的polyA尾mRNA表达谱。