Kolmancic-Zivin-Zorovic_E2_L-dopa_data2022.sav

The data are the results of behavioural testing and immunohistochemistry from a study on the effects of estrogen on the development of levodopa-induced dyskinesias in a hemiparkinsonian postmenopausal rat model. The animal study protocol was approved by the Republic of Slovenia's Administration for Food Safety, Veterinary Sector and Plant Protection (ref. no. U34401-7/2017/9). The rats were overiectomized at the start of the experiment. Two weeks following the 6-OHDA lesion of dopaminergic neurons in the right hemisphere and implantation of either empty (control) or estradiol implants, the animals started recieiving daily injections of either levodopa or saline (control). Animals were divided into four tratment groups: animals with estradiol implants and daily levodopa injections, animals with empty implants and daily levodopa injections, animals with estradiol implants and daily injections of saline and animals with empty implants and daily saline injections. Behavioural testing included tests to establish the level of levodopa-induced dyskinesias (LIDs), rotation tests (RT) and the forepaw adjusting step (FAS) test to assess the motor performance. Animal body weight was followed throughout the experiment and serum levels of estradiol were measured after euthanasia. Immunohistochemistry with anti-TH primary antibody was performed on frozen brain sections to establish the extent of the 6-OHDA lesion. We also evaluated the changes in expression of FosB protein in animals from different treatments.<br>

本数据集源自一项探究雌激素对偏侧帕金森病绝经后大鼠模型中左旋多巴诱发异动症（levodopa-induced dyskinesias，LIDs）发生发展影响的研究，涵盖行为学检测与免疫组织化学实验的全部结果。本动物实验方案已获得斯洛文尼亚共和国食品安全、兽医与植物保护局批准（编号：U34401-7/2017/9）。实验初始阶段对大鼠实施卵巢切除手术。在对大鼠右侧半球多巴胺能神经元进行6-羟基多巴胺（6-OHDA）损毁，并植入空白（对照组）或雌二醇缓释植片两周后，开始每日为各组大鼠注射左旋多巴或生理盐水（对照组）。实验动物被分为四组干预方案：雌二醇植片+每日左旋多巴注射组、空白植片+每日左旋多巴注射组、雌二醇植片+每日生理盐水注射组，以及空白植片+每日生理盐水注射组。行为学检测内容包括用于量化左旋多巴诱发异动症严重程度的评估检测、旋转实验（rotation tests，RT），以及用于评价运动功能的前爪调整步幅测试（forepaw adjusting step，FAS）。实验全程监测大鼠体重，并在安乐死后采集血清样本检测雌二醇水平。采用抗酪氨酸羟化酶（anti-TH）一抗对冰冻脑切片进行免疫组织化学染色，以确定6-羟基多巴胺（6-OHDA）神经元损毁的范围。本研究同时对不同干预组大鼠的FosB蛋白表达变化进行了检测分析。