Developing the strategy to use silk spheres for efficient, targeted delivery of oligonucleotide therapeutics to cancer cells

Data from publiaction "Developing the strategy to use silk spheres for efficient, targeted delivery of oligonucleotide therapeutics to cancer cells."Each ZIP archive contains a readme.txt file with detailed description of the files. ConfocalMicroscopy.zip - Results of the analysis of spheres internalization using confocal microscopy. FITC-Concanavalin A was used for staining cell surface carbohydrates (green), silk spheres were labeled with Cy5 (red), and nucleus was stained with DAPI (blue). Scale bar - 10 μm.PhysicochemicalProperties.zip - Results of the physicochemical analysis of spheres, including data from SEM, Zetasizer, Nanosight, and loading efficiency.FlowCytometry.zip - Results of the analysis of spheres binding to cells using flow cytometry.ProteinOligoBinding.zip - Results of the efficiency of siRNA binding to spider silk proteins.ProteinsSDSPAGE.zip - Results of SDS-PAGE gel stained with Roti Blue.qPCR.zip - Results of STAT3 silencing using siRNA delivered by silk spheres.SpheresCytotoxicity.zip - Results of the cytotoxicity analysis of spheres.

本数据集源自发表于论文《开发利用丝素微球高效靶向递送寡核苷酸疗法至癌细胞的策略》（"Developing the strategy to use silk spheres for efficient, targeted delivery of oligonucleotide therapeutics to cancer cells"）的研究数据。所有ZIP归档文件均内含readme.txt文档，详细说明该归档内所有数据集文件的相关信息。
ConfocalMicroscopy.zip：共聚焦显微镜（Confocal Microscopy）分析数据集，用于检测丝素微球的细胞内化行为。实验中以异硫氰酸荧光素标记伴刀豆球蛋白A（FITC-Concanavalin A）对细胞表面碳水化合物进行染色（荧光信号呈绿色），丝素微球经Cy5标记（荧光信号呈红色），细胞核则采用4',6-二脒基-2-苯基吲哚（DAPI）染色（荧光信号呈蓝色），图像比例尺为10 μm。
PhysicochemicalProperties.zip：丝素微球理化性质分析数据集，涵盖扫描电子显微镜（Scanning Electron Microscopy，SEM）、Zetasizer粒度分析仪、纳米颗粒跟踪分析仪（Nanosight）以及载药效率的相关检测数据。
FlowCytometry.zip：流式细胞术（Flow Cytometry）分析数据集，用于检测丝素微球与细胞的结合情况。
ProteinOligoBinding.zip：小干扰RNA（small interfering RNA，siRNA）与蛛丝蛋白结合效率的检测数据集。
ProteinsSDSPAGE.zip：经Roti Blue染色的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis，SDS-PAGE）实验数据集。
qPCR.zip：利用丝素微球递送siRNA沉默信号转导与转录激活因子3（Signal Transducer and Activator of Transcription 3，STAT3）的实时定量聚合酶链反应（quantitative Polymerase Chain Reaction，qPCR）检测数据集。
SpheresCytotoxicity.zip：丝素微球细胞毒性分析数据集。