Reproducibility Package for a Hybrid Mechanistic-Phenomenological Model Linking BoNT/A Molecular Persistence to Functional Recovery at the Neuromuscular Junction

Reproducibility package for a four-layer hybrid mechanistic-phenomenological model of botulinum neurotoxin type A (BoNT/A) at the neuromuscular junction. The model separates molecular persistence of the SNAP-25 lesion from recovery of neuromuscular output, and this deposit contains everything needed to reproduce the reported computational results: the Python implementation, the parameter-source table, the one-at-a-time robustness analysis, the literature-based consistency assessment, the Morris global sensitivity screening, the cross-species, fibre-type and repeated-injection extension analyses, all generated figures, the environment file and the checksum list. Layers 1 to 3 represent toxin entry, light-chain translocation and persistent intracellular light-chain activity with SNAP-25 cleavage. Layer 4a represents release capacity, combining capacity retained at the parent terminal with capacity supplied by sprouts and remodelled terminals. Layer 4b represents neuromuscular transduction. Endplate transmission operates with a safety factor: healthy motor nerve terminals release several times the transmitter needed to reach threshold, transmission at an individual junction is all-or-none, and the threshold floats because paralysed muscle becomes supersensitive to acetylcholine and remains so beyond the return of contraction. Junction safety factors are represented as log-normal and force is computed as the fraction of junctions in which transmission succeeds, separating two functional endpoints: the ability to contract, and fatigue-resistant output during sustained activity. No patient-level data, human-subject data or animal-experiment data were used or generated. All files are simulation-derived or model-supporting materials. Code is released under the MIT licence and all other files under CC BY 4.0. SHA256SUMS.txt lists every file in the deposit.