MAF Amplification licenses Estrogen Receptor α to Drive Breast Cancer Metastasis [ATAC-seq]

We mapped chromatin accessibility on control and MAF-overexpressing MCF7 cells to assess the consequences of estrogen (E2) and metastatic MAF expression on the chromatin landscape. To this end, we cultured MCF7 cells in hormone-deprived (HD) medium for 72 h and estrogen (E2) or vehicle was added for 1h prior to DNA purification. Samples were generated in quadruplicate. We report changes in chromaitn accessibility depending on both MAF expression and E2 stimulation. In order to assess changes in chromatin accessibility governed by MAF and estrogen (E2), control or MAF-overexpressing MCF7 cells were maintained in hormone-deprived medium for 72 h. Then, E2 or vehicle was added for 1h and DNA was purified for ATAC-seq analysis

我们对对照组及过表达MAF的MCF7细胞开展染色质可及性（chromatin accessibility）图谱分析，以探究雌激素（E2）与转移性MAF表达对染色质表观景观的影响。为此，我们将MCF7细胞置于激素剥夺（HD）培养基中培养72小时，随后添加雌激素（E2）或溶剂对照，处理1小时后进行DNA纯化。本实验设置四组生物学重复。本研究报道了同时受MAF表达与E2刺激调控的染色质可及性变化。为评估MAF与雌激素（E2）介导的染色质可及性变化，我们将对照组或过表达MAF的MCF7细胞在激素剥夺培养基中培养72小时。随后添加E2或溶剂对照处理1小时，纯化DNA以进行ATAC-seq（转座酶可及性染色质测序）分析。