Gene expression profiles of PD-L1 positive and PD-L1 negative lung neutrophils from Influenza A virus infected mice. Gene expression profiles of PD-L1 positive and PD-L1 negative lung neutrophils from Influenza A virus infected mice

Neutrophils are often considered as foes during severe lung infections through their ability to secrete inflammatory mediators and participate in lung damages. Here, we used single cell RNA-sequencing (scRNA-seq) to assess the transcriptional heterogeneity of neutrophils in mice with severe lung infection caused by influenza A virus Overall design: Lung neutrophils from IAV (H3N2/Scotland/74 strain) infected C57BL/6j femelle mice (day 11 post-infection) were isolated using a cell sorter based on PD-L1 expression.

中性粒细胞（Neutrophils）在重症肺部感染中常被视作致病因素，其可通过分泌炎性介质参与肺组织损伤。本研究采用单细胞RNA测序（single cell RNA-sequencing, scRNA-seq）技术，对甲型流感病毒诱导重症肺部感染的小鼠体内中性粒细胞的转录异质性开展评估。
实验设计：采用基于程序性死亡配体1（PD-L1）表达的细胞分选仪，分离感染甲型流感病毒（Influenza A virus, IAV）H3N2/Scotland/74毒株的C57BL/6J雌性小鼠（感染后第11天）的肺部中性粒细胞。