Table_1_Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector.pdf

Bemisia tabaci (Hemiptera: Aleyrodidae) is a highly efficient vector in the spread of chilli leaf curl virus (ChiLCV, Begomovirus) which is a major constraint in the production of chilli in South Asia. Transcriptome analysis of B. tabaci post-6 h acquisition of ChiLCV showed differential expression of 80 (29 upregulated and 51 downregulated) genes. The maximum number of DEGs are categorized under the biological processes category followed by cellular components and molecular functions. KEGG analysis of DEGs showed that the genes are involved in the functions like metabolism, signaling pathways, cellular processes, and organismal systems. The expression of highly expressed 20 genes post-ChiLCV acquisition was validated in RT-qPCR. DEGs such as cytosolic carboxypeptidase 3, dual-specificity protein phosphatase 10, 15, dynein axonemal heavy chain 17, fasciclin 2, inhibin beta chain, replication factor A protein 1, and Tob1 were found enriched and favored the virus infection and circulation in B. tabaci. The present study provides an improved understanding of the networks of molecular interactions between B. tabaci and ChiLCV. The candidate genes of B. tabaci involved in ChiLCV transmission would be novel targets for the management of the B. tabaci-begomovirus complex.

烟粉虱（Bemisia tabaci）隶属于半翅目（Hemiptera）粉虱科（Aleyrodidae），是辣椒曲叶病毒（chilli leaf curl virus，ChiLCV，菜豆金色花叶病毒属（Begomovirus））传播的高效媒介；该病毒是南亚地区辣椒生产面临的主要限制因素。研究团队对获毒6小时后的烟粉虱开展转录组分析，共鉴定出80个差异表达基因（Differentially Expressed Genes，DEGs），其中29个基因上调表达、51个基因下调表达。差异表达基因的功能分类显示，数量最多的类别为生物过程，其次为细胞组分与分子功能。对DEGs的京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析表明，这些基因参与代谢、信号通路、细胞过程及机体系统等多个功能通路。获毒后高表达的20个基因的表达水平经实时定量聚合酶链反应（Reverse Transcription Quantitative Polymerase Chain Reaction，RT-qPCR）验证。富集分析发现，胞质羧肽酶3、双特异性蛋白磷酸酶10、双特异性蛋白磷酸酶15、轴丝动力蛋白重链17、成束蛋白2、抑制素β链、复制因子A蛋白1及Tob1等差异表达基因在烟粉虱体内显著富集，且这些基因可促进病毒在烟粉虱体内的侵染与循环过程。本研究深化了对烟粉虱与辣椒曲叶病毒之间分子互作网络的认知，筛选得到的参与辣椒曲叶病毒传播的烟粉虱候选基因，可为烟粉虱-菜豆金色花叶病毒属复合病害的防控提供全新的作用靶标。