Comparative analysis the transcriptomes of the hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulator Sedum alfredii (NHE). Comparative analysis the transcriptomes of the hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulator Sedum alfredii (NHE)

The aim of this study is to investigate whether the known mechanisms of hyperaccumulaion in Brassicaceae hyperaccumulators (Arabidopsis halleri and Noccaea caerulescens) are conserved among different hyperaccumulating species or if there exist unknown mechanismsin other metal hyperaccumulating species.The comparison of transcriptomes between hyperaccumulator Sedum plumbizincicola and non-hyperaccumulator S. alfredii (NHE) was performed by taking advantage of next-generation sequencing.The data suggested that increased active uptake, long-distance transport and efficient chelation of heavy metals might represent common mechanisms occurred in various hyperaccumulators. Moreover, we found that those genes showing constitutively higher expression in S. plumbizincicola shoots are significantly enriched in processes related to cell wall metabolism, including biosynthesis/modification of cellulose, lignin, pectin, glucan, and other cell wall related components. Overall design: Roche-454 was adopted to sequence the pooled root and shoot samples of S. plumbizincicola.Illumina sequencing was used to characterize the transcriptomes of root and shoot tissues of the two Sedum species with/out 10μM Cd treatment.Biological replicates: 2 replicates for S. plumbizincicola samples.

本研究旨在探究十字花科（Brassicaceae）超富集植物（hyperaccumulator）拟南芥（Arabidopsis halleri）与天蓝遏蓝菜（Noccaea caerulescens）已知的重金属超富集机制，在不同超富集物种间是否保守，抑或其他金属超富集物种中存在未知的超富集机制。本研究通过高通量测序（next-generation sequencing）技术，开展了超富集植物伴矿景天（Sedum plumbizincicola）与非超富集型东南景天（S. alfredii，简称NHE）的转录组（transcriptome）比较分析。研究数据表明，重金属的主动摄取增强、长距离转运及高效螯合作用，或许是各类超富集植物共有的重金属超富集机制。此外，本研究发现，伴矿景天地上组织中组成型高表达的基因，显著富集于细胞壁代谢相关生物学过程，涵盖纤维素、木质素、果胶、葡聚糖及其他细胞壁相关组分的生物合成与修饰。总体实验设计如下：采用罗氏454（Roche-454）测序平台，对伴矿景天的混合根、地上组织样本进行测序；利用Illumina测序技术，对两种景天属植物在添加/不添加10μM镉（Cd）处理下的根、地上组织的转录组进行表征分析。生物学重复设置：伴矿景天样本设置2次生物学重复。