SuperSAGE analysis between nitrogen fertilized samples and control in Fagus crenata. Fagus crenata

SuperSAGE library for sequencing using Illumina Hiseq2000 were generated from the nitrogen fertilized samples and control of Fagus crenata. The SuperSAGE tags were mapped to the contig sequences of the RNA-seq assembly. RPM(Read Per Million mapped reads) values of each gene in the samples were calculated to quantify gene expression and assess differential expression.

本数据集的超级基因表达系列分析（SuperSAGE）文库采用Illumina Hiseq2000测序平台构建，其样本取自日本水青冈（Fagus crenata）的氮肥处理组与对照组。将获得的SuperSAGE标签比对至RNA-seq组装得到的重叠群（contig）序列，随后计算样本中各基因的RPM（每百万比对读段数，Read Per Million mapped reads）值，以此量化基因表达水平并评估差异表达情况。