MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in human cancer [RNA-seq]

The effect of gene perturbation on the transcriptome was evaluated in human Cas9 expressing cancer cell lines Human pancreatic cancer cell lines were cultured in DMEM,RPMI or HITES supplemented with 5 or 10% fetal bovine serum (FBS). RNA-extraction (Triazol) and Poly-A followed by library preparation was performed in accordance to manufacturer’s instructions (Illumina Truseq Kit).

本研究在表达Cas9的人类癌细胞系中，评估了基因扰动对转录组的影响。实验所用的人类胰腺癌细胞系采用添加了5%或10%胎牛血清（FBS, Fetal Bovine Serum）的DMEM、RPMI或HITES培养基进行培养。随后严格遵循Illumina Truseq试剂盒的厂商操作指南，完成了TRIzol法RNA提取、Poly-A富集及文库构建流程。