A comprehensive pipeline for the transcriptome analysis of Puccinia kuehnii, the sugarcane orange rust fungal pathogen, provides insights into the early and late molecular responses during compatible and incompatible host interactions

Puccinia kuehnii, the causal agent of orange rust, has caused significant losses to the sugarcane crop in Florida since 2007. During plant infection, rust fungi deliver effector proteins into host tissues to promote parasitic growth while plants activate their immune systems to respond to this invasion. The main goal of this research was to analyze the transcriptome of P. kuehnii during time-course infections of sugarcane, including compatible and incompatible interactions.

昆士兰柄锈菌（Puccinia kuehnii）作为橙色锈病的致病菌，自2007年起已对美国佛罗里达州的甘蔗产业造成了严重经济损失。在植物侵染过程中，锈菌会将效应蛋白（effector protein）转运至宿主组织中，以促进其寄生生长；而宿主植物则会激活自身免疫系统以应对该侵染。本研究的核心目标为：分析昆士兰柄锈菌在甘蔗时序侵染过程中的转录组（transcriptome）特征，实验涵盖亲和互作与非亲和互作两种互作类型。