RNA sequencing analysis for keratinocytes treated with U1 dsRNA and LL34 Alanine Scan Peptides. RNA sequencing analysis for keratinocytes treated with U1 dsRNA and LL34 Alanine Scan Peptides

Summary: We looked at the global changes in gene expression in keratinlcytes treated with selected LL34 Alanine mutants and U1 dsRNA. Methods: Normal human keratinocytes were treated with 2.5 ug/ml U1 dsRNA with or with out co-treatment with LL34 Parent Peptide/ LL34 I24A or LL34 L31A for 18 h. Conclusions: Global gene expression analysis showed pathways related to Type 1 interferon signaling and cytokines where mainly repressed in cells co-treated with either LL34 I24A/ LL34 L31A peptide and U1 dsRNA in comparision to cells co-treated with LL34-Parent peptide and U1 dsRNA. Overall design: Pooled samples from triplicates were used for RNA seq analysis.

摘要：本研究旨在探究经筛选的LL34丙氨酸突变体与U1双链RNA（U1 dsRNA）处理后人角质形成细胞（keratinocyte）的基因表达全局变化。
方法：将正常人角质形成细胞以2.5 μg/ml的U1 dsRNA进行处理，同时添加或不添加LL34亲本肽、LL34 I24A或LL34 L31A进行共处理，培养时长为18小时。
结论：全局基因表达分析结果表明，相较于仅联合LL34亲本肽与U1 dsRNA处理的细胞，联合LL34 I24A/LL34 L31A肽与U1 dsRNA处理的细胞中，与Ⅰ型干扰素（Type 1 interferon）信号通路及细胞因子相关的通路整体受到显著抑制。
整体设计：本研究采用三次生物学重复的混合样本开展RNA测序（RNA-seq）分析。