Comprehensive analysis of adrenocortical steroidogenesis in human and mouse. Comprehensive analysis of adrenocortical steroidogenesis in human and mouse

We analyze gene regulatory mechanisms involved in adrenocortical steroidogenesis using assays to measure RNA expression, synthesis, decay, processing, translation and miRNA expression. Overall design: Metabolic labeling, ribosome profiling and small RNA-sequencing was performed on human H295R adrenocortical cell lines with and without treatment with angiotensin or forskolin. RNA-seq was performed on adrenal glands of p15 c57bl/6 mice.

本研究通过开展检测RNA表达、合成、降解、加工、翻译及microRNA (miRNA)表达的相关实验，分析参与肾上腺皮质类固醇生成的基因调控机制。整体实验设计：在经血管紧张素或佛司可林处理与未处理的人源H295R肾上腺皮质细胞系中，开展代谢标记、核糖体谱分析及小RNA测序实验；同时对p15日龄C57BL/6小鼠的肾上腺组织开展RNA测序（RNA-seq）实验。