SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in October 2014

Flow cytometry data was collected in October 2014, in waters off South Australia. \n\n\nThe general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.\n\nSix main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), B4 (35.168S, 135.418E) and B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

流式细胞术（Flow cytometry）数据于2014年10月在南澳大利亚附近海域采集。

本研究的总体目标是建立南澳大利亚大陆架生态系统的背景知识，阐明上升流/盐水流出事件对微生物群落的影响，最终构建该区域的生物地球化学模型。采样工作在Ngerin号考察船（RV Ngerin）的航次中进行，属于南澳大利亚综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）项目的一部分。每次航次均对叶绿素荧光最大值（DCM）层的物理、化学及生物特性展开调查。研究采集了微微型浮游植物、细菌和病毒的流式细胞术数据。

研究期间共对6个主要站点进行采样：其中5个位于100米等深线处，即RS站（35.508°S，136.278°E）、B2站（35.418°S，136.148°E）、B3站（35.258°S，136.048°E）、B4站（35.168°S，135.418°E）及B5站（35.008°S，135.198°E）；另有1个为离岸站点B1（36.188°S，136.178°E），位于袋鼠岛西南方向。需注意，结合站点间距离（14–25海里）、陆架中层海流速度平均分量（0.01 m/s）及船舶平均航速（9节）分析可知，各站点采集的水样分属不同水团。此外，研究偶尔还从袋鼠岛国家参考站（NRS，35.832°S，136.447°E）及南澳斯宾塞湾口锚系站（SAM8SG，35.25°S，136.690°E）采集样本，后者为盐水流出发生地。