Expression data from wild-type (WT) and lrrc19 gene knockout (KO) mice intestines

LRRC19 (Leucine repeat receptor containing 19) is a newfound Toll-like receptor without definite function reported. We have detected that the lrrc19 gene had a high expression level in both human and mouse intestines and concentrated on the effect of this immune receptor in mediating the process of enteritis and intestinal cancer. To have a systematic understanding on the influence of this gene on the intracelluar environment of intestinal cell, we have conducted a microarray experiment to compare the transcriptomes of the WT and KO mice intestines under the same experimental condition. Gut epithelial cells of WT and KO mice of the same physiological and maintaining condition were isolated and then total RNAs were extracted from them, which could reflect the respective transcription profile of WT and KO mice. These RNAs were detected by microarray and the influence of lrrc19 gene on intestine could be revealed to some extent.

LRRC19（含亮氨酸重复序列受体19，Leucine repeat receptor containing 19）是一种新近发现、功能尚未明确的Toll样受体（Toll-like receptor）。
本研究团队已检测到，lrrc19基因在人类与小鼠肠道中均呈高表达状态，并聚焦于该免疫受体介导肠炎与肠癌进程的功能研究。
为系统解析该基因对肠道细胞胞内微环境的影响，本研究开展了基因芯片实验，在统一实验条件下对比野生型（Wild Type, WT）与敲除型（Knock Out, KO）小鼠肠道的转录组差异。
研究人员分离得到生理状态与饲养条件一致的野生型与敲除型小鼠肠道上皮细胞，并从中提取总RNA，以反映两类小鼠各自的转录谱特征。
通过基因芯片检测上述总RNA，可在一定程度上揭示lrrc19基因对肠道的调控影响。