Identification and Analysis of Shared Genes and Pathways in Osteoarthritis and Sarcopenia Patients: Supplementary Figures and Tables

Supplementary Figure 1. Data sets preprocessing. (A and B) Boxplot of GSE114007 dataset before and after normalization; (C and D) boxplot of GSE111017 before and after normalization.Supplementary Figure 2. Screening of Candidate Hub Genes. A: PPI network constructed from the 89 common DEGs. B: selection of 17 candidate hub genes using Cytoscape software.Supplementary Figure 3. Immune cell infiltration. A: Differentially expressed immune cells compared with OA and normal controls. B: Differentially expressed immune cells compared with sarcopenia and normal controls. C: Correlation between AEBP1, COL8A2 and 28 immune cells in OA samples. D: Correlation between AEBP1, COL8A2 and 28 immune cells in sarcopenia samples. Supplementary Table 1. Primer sequences of AEBP1 and COL8A2.Supplementary Table 2.The comprehensive results of GO and KEGG enrichment analyses.Supplementary Table 3.Detailed results of the PPI network construction and CytoHubba analysis.Supplementary Table 4.The detailed results of the GSEA.

补充图1 数据集预处理。(A、B) GSE114007数据集标准化前后的箱线图；(C、D) GSE111017数据集标准化前后的箱线图。补充图2 候选核心基因筛选。A：由89个共同差异表达基因（Differentially Expressed Genes, DEGs）构建的蛋白质相互作用（Protein-Protein Interaction, PPI）网络；B：通过Cytoscape软件筛选得到17个候选核心基因。补充图3 免疫细胞浸润分析。A：骨关节炎（Osteoarthritis, OA）与正常对照组的差异表达免疫细胞；B：肌肉减少症（Sarcopenia）与正常对照组的差异表达免疫细胞；C：骨关节炎样本中AEBP1、COL8A2与28种免疫细胞的相关性；D：肌肉减少症样本中AEBP1、COL8A2与28种免疫细胞的相关性。补充表1：AEBP1与COL8A2的引物序列。补充表2：基因本体（Gene Ontology, GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析的综合结果。补充表3：PPI网络构建及CytoHubba分析的详细结果。补充表4：基因集富集分析（Gene Set Enrichment Analysis, GSEA）的详细结果。