Modeling Glioma Oncostreams In Vitro: Spatiotemporal Dynamics of their Formation, Stability, and Disassembly

Movie 1 of 16 is a supplementary material for the manuscript 'Modeling Glioma Oncostreams In Vitro: Spatiotemporal Dynamics of their Formation, Stability, and Disassembly.' It visually demonstrates the effect of various treatments on the formation and dynamics of oncostreams in high-grade glioma cells. The time-lapse videos provide insightful observations into how various pharmacological drugs influence the initial formation and structural dynamics of oncostreams."

It aims to enhance the understanding of the foundational stages of oncostream formation in glioma cells and their response to various pharmacological treatments, supporting the findings discussed in the manuscript.

Individual Movie Descriptions:

Movie 1: Low Density Oncostream Dynamics: Demonstrates the formation and dynamics of oncostreams in GFP+ NPA glioma cells at low seeding density (1 × 10^5 cells), over 24 hours using time-lapse confocal imaging.

Movie 2: High Density Oncostream Dynamics: Explores the effect of high cell seeding density (2 × 10^5 cells) on the formation and behavior of oncostreams in GFP+ NPA glioma cells, analyzed over 24 hours.

Movie 3: Spatiotemporal Progression of Oncostreams: Showcases the sequential self-formation and developmental stages of oncostreams over a 24-hour period.

Movie 4: Collagenase Impact on Oncostreams: Description: Illustrates the disassembly of aggressive and malignant oncostreams with 15 U/ml collagenase treatment over 15 hours.

Movie 5: TC-I-15 Inhibition of Oncostream Formation: Details the impact of TC-I-15, an integrin antagonist, on adhesion and oncostream formation in glioma cells, recorded over 20 hours.

Movie 6: Cytochalasin D Disruption of Oncostreams: Captures the effect of Cytochalasin D on oncostream formation by inhibiting actin polymerization, observed over a 1-hour period with rapid imaging intervals.

Movie 7: Myosin II Inhibition in Oncostreams with p-nitro Blebbistatin: Presents the influence of p-nitro Blebbistatin on oncostream formation by inhibiting myosin II, documented over 1 hour with dynamic cellular responses.

Movie 8: Control - Oncostream Formation without BAPTA-AM: Demonstrates oncostream formation and dynamics without BAPTA-AM treatment, monitored over 16 hours.

Movie 9: BAPTA-AM (Calcium Modulation) Impact on Oncostream Formation: Reveals the effects of BAPTA-AM treatment on oncostream dynamics, observed over a 16-hour period.

Movie 10: Glutamate Influence on Oncostreams: Shows the effects of glutamate treatment on oncostream formation and dynamics, monitored over 16 hours.

Movie 11: Histamine Impact on Oncostream Dynamics: Demonstrates the influence of histamine on the formation and behavior of oncostreams, captured over a 16-hour period.

Movie 12: Oncostream Formation on Non-Laminin Coated Surfaces: Illustrates the compromised organization of oncostreams on poly-D-lysine coated dishes without laminin, observed over 45 hours.

Movie 13: 4-HAP Treatment Effect on Oncostreams: Shows the impact of 4-HAP treatment on the structure of oncostreams, monitored over 20 hours.

Movie 14: Rho-Activator I Untreated Control: Presents the formation and dynamics of oncostreams without Rho-Activator I treatment, recorded over 16 hours.

Movie 15: Rho-Activator I Influence on Oncostreams Dynamics: Details the effects of Rho-Activator I on oncostream formation and dynamics, observed over a 16-hour period.

Movie 16: Rho-Inhibitor Effect on Oncostreams Dynamics: Demonstrates the impact of Rho-Inhibitor on the formation and behavior of oncostreams, monitored over 16 hours.

本数据集包含的16部影片均为学术论文《体外建模胶质瘤癌流（Glioma Oncostreams）：其形成、稳定性与解体的时空动力学》的补充材料。该数据集可视化呈现了不同处理方式对高级别胶质瘤细胞中癌流形成与动力学过程的影响。延时成像视频可为探究各类药理学药物如何影响癌流的初始形成与结构动力学提供富有洞见的观测视角。

本数据集旨在加深对胶质瘤细胞中癌流形成基础阶段及其对各类药理学药物响应的理解，以佐证论文中阐述的研究结论。

单部影片详情：

影片1：低接种密度癌流动力学：采用共聚焦延时成像技术，对低接种密度（1×10^5个细胞）下GFP标记的NPA胶质瘤细胞的癌流形成与动力学过程进行24小时观测。
影片2：高接种密度癌流动力学：探究高细胞接种密度（2×10^5个细胞）对GFP标记的NPA胶质瘤细胞中癌流形成与行为的影响，观测时长为24小时。
影片3：癌流的时空进展：展示癌流在24小时内的连续自我形成与发育阶段。
影片4：胶原酶（Collagenase）对癌流的影响：展示使用15 U/ml胶原酶处理后，侵袭性恶性癌流的解体过程，观测时长为15小时。
影片5：TC-I-15抑制癌流形成：详述整联蛋白拮抗剂TC-I-15对胶质瘤细胞黏附与癌流形成的影响，记录时长为20小时。
影片6：细胞松弛素D（Cytochalasin D）破坏癌流：记录细胞松弛素D通过抑制肌动蛋白聚合对癌流形成的影响，采用快速成像间隔开展1小时观测。
影片7：p-硝基 Blebbistatin 抑制肌球蛋白II（Myosin II）对癌流的影响：展示p-硝基 Blebbistatin 通过抑制肌球蛋白II对癌流形成的影响，记录时长为1小时，同步观测细胞动态响应。
影片8：对照组：无BAPTA-AM处理的癌流形成：展示未经过BAPTA-AM处理的癌流形成与动力学过程，监测时长为16小时。
影片9：BAPTA-AM（钙离子调控）对癌流形成的影响：展示BAPTA-AM处理对癌流动力学的影响，观测时长为16小时。
影片10：谷氨酸（Glutamate）对癌流的影响：展示谷氨酸处理对癌流形成与动力学的影响，监测时长为16小时。
影片11：组胺（Histamine）对癌流动力学的影响：展示组胺对癌流形成与行为的影响，记录时长为16小时。
影片12：非层粘连蛋白包被表面上的癌流形成：展示在未包被层粘连蛋白的多聚赖氨酸培养皿中，癌流组织形态受损的情况，观测时长为45小时。
影片13：4-HAP处理对癌流的影响：展示4-HAP处理对癌流结构的影响，监测时长为20小时。
影片14：Rho激活剂I（Rho-Activator I）未处理对照组：展示未经过Rho激活剂I处理的癌流形成与动力学过程，记录时长为16小时。
影片15：Rho激活剂I对癌流动力学的影响：详述Rho激活剂I对癌流形成与动力学的影响，观测时长为16小时。
影片16：Rho抑制剂（Rho-Inhibitor）对癌流动力学的影响：展示Rho抑制剂对癌流形成与行为的影响，监测时长为16小时。