Lupane triterpenes from the leaves of the tropical rain forest tree Hopea odorata Roxb. and their cytotoxic activities, supplementary information

Chromatographic fractionation of the cytotoxic n-hexane extract of Hopea odorata Roxb. leaves led to the isolation of eight lupane triterpenes, which constitutes the first report of lupane-type triterpenes from this plant source. Furthermore, 3,30-dioxolup-20(29)-en-28-oic acid (6) was isolated for the first time from a natural source. Their structures were determined on the basis of spectroscopic methods, including 2D NMR analysis, and by comparison of their spectral data with literature values. Complete NMR assignments of the 1H and 13C NMR data were achieved for all compounds. Finally, the cytotoxic activities of the isolated compounds against four human cell lines (PC3, MDA-MB-231, HT-29 and HCT116) was also reported.

对香坡垒（Hopea odorata Roxb.）叶片的细胞毒性正己烷提取物进行色谱分离，成功获得8个羽扇豆烷型三萜（lupane triterpenes），此为首次从该植物中报道羽扇豆烷型三萜类成分。此外，3,30-二氧羽扇豆-20(29)-烯-28-酸（6）系首次从天然来源中分离得到。通过波谱学技术（包括二维核磁共振（2D NMR）分析），并将其光谱数据与文献值对比，确定了所有化合物的结构；同时完成了各化合物1H和13C核磁共振数据的完整归属。最后，还报道了这些化合物对PC3、MDA-MB-231、HT-29及HCT116四种人类细胞系的细胞毒性活性。