Evaluation Of Peanut Skin Ethanol Extract As Protective Agent Against Lead Acetate Induced Reproductive And Neuromuscular Toxicities In Drosophila Melanogaster

Lead acetate exposure is known to cause reproductive and locomotor toxicities in various organisms, including Drosophila melanogaster. This study aimed to assess the ameliorative effects of peanut skin ethanol extract (PSE) against lead acetate-induced toxicities in Drosophila melanogaster. Fly body weight, larval locomotor activity, fecundity, and eclosion activity were evaluated as endpoints. Lead acetate exposure reduced fly body weight, larval locomotor activity, and fecundity, and hindered the eclosion of adult flies. Co-treatment with PSE at different concentrations (LP1, LP2, LP3) further exacerbated these toxic effects, except when co-administered with resveratrol (LR), which mitigated the lead-induced locomotor dysfunction and increased fecundity. Co-treatment with PSE (LP1, LP3) or PSE alone (P1, P2, P3) exceptionally mitigated lead-induced weight loss and increased the fly body weight. Treatment with PSE alone showed a reduction in larval locomotor activity and did not significantly affect fecundity or eclosion. The observed effects may be attributed to the fatty acid content of the peanut skin extract, sensitivity of neuronal pathways, and potential interactions with lead. These findings suggest that peanut skin ethanol extract may have limited protective effects against lead-induced toxicities in Drosophila melanogaster, and further investigations are warranted to understand the underlying mechanisms and potential implications for human health.

乙酸铅暴露已知会对包括黑腹果蝇（Drosophila melanogaster）在内的多种生物造成生殖毒性和运动毒性。本研究旨在评估花生皮乙醇提取物（PSE）对乙酸铅诱导的黑腹果蝇毒性的缓解作用，以果蝇体重、幼虫运动活性、繁殖力和羽化活性为评估终点。乙酸铅暴露降低了果蝇体重、幼虫运动活性与繁殖力，并阻碍成虫羽化。不同浓度PSE（LP1、LP2、LP3）联合处理进一步加剧了这些毒性效应，但与白藜芦醇（LR）联用除外——LR可缓解乙酸铅诱导的运动功能障碍并提高繁殖力。值得注意的是，PSE（LP1、LP3）联合处理或单独PSE处理（P1、P2、P3）能缓解乙酸铅诱导的体重下降并提升果蝇体重。单独PSE处理会降低幼虫运动活性，但对繁殖力或羽化无显著影响。观察到的效应可能与花生皮提取物中的脂肪酸含量、神经通路敏感性及与铅的潜在相互作用有关。这些发现表明，花生皮乙醇提取物对乙酸铅诱导的黑腹果蝇毒性保护作用有限，需进一步研究阐明其潜在机制及对人类健康的可能意义。