Diurnal RNA-Seq for Petunia hybrida

To better understand how diurnal transcriptional regulation contributes to day/night cycles of volatile emission from the petunia flower, RNA extracted from corolla in the morning (7AM) and evening (7PM) was sequenced to determine genes differentially expressed at the two time points. Three replicates of RNA were sequenced and aligned to the Petunia axillaris reference genome to determine transcript levels at 7AM and 7PM.

为深入探究昼夜转录调控如何介导矮牵牛（Petunia axillaris）花朵挥发性物质释放的昼夜节律，本研究分别于早晨7点与傍晚7点从花冠组织中提取RNA并进行测序，以鉴定两个时间点间的差异表达基因。本次实验共设置三次生物学重复，将测序所得序列比对至矮牵牛参考基因组，以此量化早晨7点与傍晚7点的转录本表达水平。