The effect of acute and chronic temperature stress in Baikal omul (Coregonus migratorius Georgi)

In this work, we describe the transcriptional profiles of Baikal omul juveniles after acute and chronic temperature stress exposure. The juveniles were kept for 1.5 months at 9–12 °C, followed by exposure to acute stress (heating to 21 °C for 1 hour) and chronic stress (heating to 21 °C for 24 hours 3 times a week for a month) in the Experimental Freshwater Aquarium Complex for Baikal Hydrobionts at the Limnological Institute (LIN SB RAS). The information on the transcriptional profiles will contribute to further understanding of the mechanisms of adaptation of whitefish to the environment. Overall design: The experiment aims to study the effects of acute and chronic stress on aquaculture larvae of Baikal omul (Coregonus migratorius Georgi). The individuals were collected from ponds near the Belaya River (Belsky fish hatchery, Sosnovka village, Irkutsk region, 52 ° 50'05.4"N 103 ° 22'51.8"E). Individuals were kept for 1.5 months in aquaculture at a temperature of 9-12 ° C with subsequent heat treatment: acute stress implied rapid heating to 21 ° C for 1 hour, chronic stress implied heating to 21 ° C for 24 hours 3 times a week. Grant ID No.: RSF 23-24-00644 ( https://rscf.ru/project/23-24-00644/ ) Grant title: Biomarkers of temperature stress and indicators of the well-being of whitefish in the natural environment and in aquaculture for the further use of these biomarkers for monitoring work. Funding source: Russian Science Foundation

本研究阐述了贝加尔白鲑幼鱼经急性与慢性温度胁迫暴露后的基因转录谱。实验所用幼鱼于俄罗斯科学院西伯利亚分院湖沼研究所（LIN SB RAS）的贝加尔水生生物专用实验淡水水族系统中，先在9~12 ℃条件下驯养1.5个月，随后分别施加急性温度胁迫（快速升温至21 ℃并持续1小时）与慢性温度胁迫（每周3次升温至21 ℃并持续24小时，共持续1个月）。

该基因转录谱数据将为深入解析白鲑的环境适应机制提供重要支撑。

实验设计概况：本实验旨在探究温度胁迫对贝加尔白鲑（Coregonus migratorius Georgi）养殖幼体的影响。实验个体采自别拉亚河沿岸养殖池塘（俄罗斯伊尔库茨克州索斯诺夫卡村别尔斯基鱼苗场，坐标：52°50'05.4"N，103°22'51.8"E）。

实验个体先于养殖系统中以9~12 ℃水温驯养1.5个月，随后开展热处理：急性胁迫组为快速升温至21 ℃并持续1小时，慢性胁迫组为每周3次升温至21 ℃并持续24小时。

资助信息：
资助编号：RSF 23-24-00644（https://rscf.ru/project/23-24-00644/）
资助项目标题：自然环境与水产养殖环境下白鲑温度胁迫生物标志物及健康状态指标研究，以推动此类标志物在监测工作中的应用
资助方：俄罗斯科学基金会（Russian Science Foundation）