Fertilization success rates from gamete age assays using eggs and sperm from Porites lobata corals in April-May 2023

This dataset includes fertilization success rates from gamete age assays using eggs and sperm from Porites lobata corals in April-May 2023. Porites lobata colonies were collected from study sites in the Rock Islands Southern Lagoon in the Republic of Palau. Colonies were isolated in individual plastic containers at the Palau International Coral Reef Center. Sperm from selected male colonies were pooled in a single container, and eggs were gently poured into this pool, allowing fertilization to commence. Fertilization success was evaluated visually 2-3 hours after fertilization began by observing a sub-sample of the eggs from each cross under a dissecting microscope and counting whole (undivided) eggs and dividing embryos.

本数据集涵盖2023年4月至5月开展的配子年龄试验所得的受精成功率数据，试验材料采自叶状微孔珊瑚（Porites lobata）的卵细胞与精子。叶状微孔珊瑚群落采集自帕劳共和国洛克群岛南部潟湖的研究样地，所有采集的珊瑚群落均在帕劳国际珊瑚礁中心的独立塑料容器中进行隔离培养。将筛选出的雄性珊瑚群落的精子混合于单一容器中，随后轻柔地将卵细胞倒入该混合液中，启动受精过程。受精启动后2至3小时，通过体视显微镜观察每个杂交组合的卵细胞子样本，计数完整（未分裂）卵细胞与分裂胚胎的数量，以此视觉评估受精成功率。