RNA-Seq analysis of isolated myeloid cells and melanoma cells, B16F10 and YUMM3.3.. RNA-Seq analysis of isolated myeloid cells and melanoma cells, B16F10 and YUMM3.3.

We evaluated the changes in the transcriptome of spleen and bone marrow isolated myeloid cells in healthy and melanoma bearing animals and melanoma cell lines, B16F10 and YUMM3.3 using RNA-sequencing technologies. Overall design: mRNA profile changes comparing myeloid cells in healthy compared to melanoma-bearing animals and comparing melanoma cell lines B16F10 and YUMM3.3.

本研究采用RNA测序（RNA-sequencing）技术，对健康动物、荷黑色素瘤动物体内分离得到的脾脏与骨髓髓系细胞（myeloid cells），以及黑色素瘤细胞系B16F10和YUMM3.3的转录组（transcriptome）变化进行了评估。实验设计概述：对比健康动物与荷黑色素瘤动物的髓系细胞mRNA表达谱变化，同时对比黑色素瘤细胞系B16F10与YUMM3.3的mRNA表达谱差异。