Genomic and in situ analyses reveal the Micropruina spp. as abundant fermentative glycogen accumulating organism in enhanced biological phosphorus removal systems. Genome sequence of Micropruina glycogenica Lg2

Enhanced biological phosphorus removal (EBPR) configurations, for the treatment of wastewater, involve the cycling of the biomass through carbon-rich (feast) and carbon-deficient (famine) conditions promoting the activity of polyphosphate accumulating organisms (PAO). However, several alternate metabolic strategies, without polyphosphate storage, are possessed by other organisms to compete with the PAO for carbon at the potential expense of EBPR efficiency. The most studied are the glycogen accumulating organisms (GAO), which utilise aerobically stored glycogen to energise anaerobic substrate uptake and storage. In a recent 16S rRNA amplicon survey of full-scale EBPR plants in Denmark, Micropruina spp. were identified as the most abundant of the previously proposed GAO, yet little is known about their ecophysiology. In the current study, genomic and metabolomic studies were made of Micropruina glycogenica str. Lg2T and compared to the in situ physiology of the genus in EBPR plants using state-of-the-art single cell techniques.

强化生物除磷（Enhanced Biological Phosphorus Removal, EBPR）工艺用于废水处理，通过使微生物菌群在富碳（饱食）与碳匮乏（饥饿）条件下循环，促进聚磷菌（polyphosphate accumulating organisms, PAO）的代谢活性。然而，部分其他微生物可通过无需聚磷储存的替代代谢策略，与聚磷菌竞争碳源，且可能降低EBPR的处理效率。其中研究最为广泛的为糖原累积菌（glycogen accumulating organisms, GAO），此类微生物利用好氧阶段储存的糖原为厌氧阶段的底物摄取与储存过程供能。近期一项针对丹麦全规模EBPR污水处理厂的16S rRNA扩增子测序调查显示，微普鲁菌属（Micropruina spp.）被鉴定为此前提出的糖原累积菌中丰度最高的类群，但目前对其生态生理学特性的了解仍十分有限。本研究对糖原微普鲁菌（Micropruina glycogenica）菌株Lg2T开展了基因组学与代谢组学研究，并借助前沿单细胞技术，将该菌株的生理特性与EBPR污水处理厂中该属微生物的原位生理状态进行了对比分析。