Transposon_directed_insertion_site_sequencing_of_ISS1_libraries_of_Streptococcus_equi_recovered_from_ponies_with_strangles

We hypothesise that surface proteins of S. equi, which can be targeted for vaccine design, play an important role in its ability to cause strangles in horses. Thus, our aim is to identify S. equi genes that are required for the infection of Welsh mountain ponies, greatly increasing our knowledge of this important equine pathogen. Objectives are to infect 15 Welsh mountain ponies with three tagged ISS1 mutant library of S. equi and recover abscess material for sequencing; to identify ISS1 mutants in the challenge inoculum and abscess material recovered from ponies post-challenge; to determine the fitness of individual ISS1 mutants by comparing the population of mutants sequenced pre- and post-challenge.我们提出假说：可作为疫苗研发靶点的马腺疫链球菌（Streptococcus equi）表面蛋白，在其引发马腺疫的过程中发挥关键作用。据此，本研究旨在鉴定出马腺疫链球菌感染威尔士山地矮马所必需的基因，以显著深化我们对这一重要马源病原菌的认知。本研究的目标包括：使用三套带有标签的马腺疫链球菌ISS1突变体文库感染15匹威尔士山地矮马，并采集脓肿样本用于测序；鉴定攻毒接种物以及攻毒后从矮马体内回收的脓肿样本中的ISS1突变体；通过对比攻毒前后测序得到的突变体种群丰度，确定单个ISS1突变体的适合度。