The effect of LAIR-1 ligation on naïve CD8 T cells

To assess the role of LAIR-1 signalling on CD3 stimulated CD8 T cells, we ligated LAIR-1 on CD8 T cells during CD3 mediated CD8 T cell stimulation naïve CD8 T cells were isolated and cultured for 6 hours in the presence of anti-CD3, or corresponding isotype antibody, and anti-LAIR-1 (or isotype control)

为评估白细胞相关免疫球蛋白样受体1（LAIR-1）信号通路在经CD3刺激的CD8 T细胞中的作用，我们在CD3介导的CD8 T细胞刺激过程中，对CD8 T细胞表面的LAIR-1进行交联。本实验分离得到初始CD8 T细胞，并将其在添加了抗CD3抗体或相应同型对照抗体、以及抗LAIR-1抗体（或同型对照）的培养液中培养6小时。