Sequences in Influenza A Virus PB2 Protein That Determine Productive Infection for an Avian Influenza Virus in Mouse and Human Cell Lines

Reverse genetics was used to analyze the host range of two avian influenza viruses which differ in their ability to replicate in mouse and human cells in culture. Engineered viruses carrying sequences encoding amino acids 362 to 581 of PB2 from a host range variant productively infect mouse and human cells.

本研究采用反向遗传学（Reverse genetics）技术，分析两株在体外培养的小鼠细胞与人类细胞中复制能力存在差异的禽流感病毒的宿主范围（host range）。携带源自宿主范围变异株的PB2蛋白氨基酸362至581编码序列的工程化病毒，可增殖性感染小鼠与人类细胞。