Pilot-Scale Depuration Trial Data

We conducted several depuration trials to reduce <i>Vibrio parahaemolyticus </i>in three species of oyster: <i>Crassostrea gigas</i>, <i>C. sikamea</i>, and <i>C. virginica</i>. We also compared depuration of non-pathogenic and pathogenic isolates of <i>V. parahaemolyticus.</i> Depuration trials were conducted at 11 or 13 °C for 5-7 days, with oysters sampled daily to evaluate <i>V. parahaemolyticus</i> concentrations. Oysters were homogenized and the homogenate cultured to evaluate <i>V. parahaemolyticus </i>concentrations. Reductions in <i>V. parahaemolyticus</i> were determined by plating homogenized oyster tissue on TCBS agar, a selective media for <i>Vibrio</i>.Depuration significantly reduced the concentrations of <i>V. parahaemolyticus</i> in all three species of oyster tested, and non-pathogenic and pathogenic <i>V. parahaemolyticus</i> isolates were reduced at an equivalent rate, allowing for their use in future depuration validation studies.

本研究开展多组净化试验，旨在降低三种供试牡蛎体内的副溶血性弧菌（Vibrio parahaemolyticus）载量，供试物种分别为长牡蛎（Crassostrea gigas）、相模湾牡蛎（C. sikamea）与美洲牡蛎（C. virginica）。同时，本研究还对比了副溶血性弧菌非致病分离株与致病分离株的净化效果差异。试验设置11℃与13℃两个温度梯度，持续处理5至7天，每日采集牡蛎样本以检测副溶血性弧菌浓度。将采集的牡蛎组织进行匀浆后，对匀浆液进行培养以定量副溶血性弧菌载量；副溶血性弧菌的消减水平通过将匀浆组织涂布于弧菌选择性培养基——硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂（TCBS agar）上进行测定。
试验结果显示，净化处理可显著降低三种供试牡蛎体内的副溶血性弧菌载量，且非致病株与致病株的消减速率无显著差异，该分离株可用于后续的净化验证研究。