Additional file 1 of Single-cell genomic and transcriptomic landscapes of primary and metastatic colorectal cancer tumors

Additional file 1: Table S1. Basic information of sample cohort. N, adjacent normal tissue. PT, primary tumor. LyM, lymph node metastasis. LM, liver metastasis. OM, omentummetastasis. LN, adjacent normal tissue collected from liver. Table S2. DEGs between normal epithelial cells and tumor epithelial cells. Related to Fig. 3A Table S3. Cell viability of organoids and DEGs between control and FH534-treated organoids. Related to Fig. 2. Table S4. Mitochondrial mutations of each patient. Only the sites where mutations were detected in at least two cells were listed in the table. WT, wild type. Undet, reads than least than 9X. Heter, heterozygous mutation. Homo, homozygous. T and N represent tumor and normal. Table S5. DEGs between OLFM4+SOX9- and OLFM4+SOX9+ tumor cells. Related to Fig. S10A. Table S6. Single-cell targeted cDNA Sanger sequencing result. The number inside the box represents the number of the cell barcode. Green, orange and red colors represent wild type, heterozygous mutant and homozygous mutant respectively. No color fill represents undetected. Table S7. DEGs between BRAFV600E and BRAF600WT tumor cells. Related to Fig. S10C-D. Table S8. DEGs between KRASWT and KRASmut tumor cells. Related to Fig. S10H-I. Table S9. Somatic mutations of samples. Table S10. Antibody information and sequences of primers.

附加文件1：表S1。样本队列基本信息。其中：N为癌旁正常组织（adjacent normal tissue），PT为原发肿瘤（primary tumor），LyM为淋巴结转移（lymph node metastasis），LM为肝转移（liver metastasis），OM为大网膜转移（omentum metastasis），LN为取自肝脏的癌旁正常组织（adjacent normal tissue collected from liver）。
表S2：正常上皮细胞与肿瘤上皮细胞间的差异表达基因（differentially expressed genes, DEGs），对应图3A。
表S3：类器官（organoids）的细胞活力，以及对照组与FH534处理组类器官间的差异表达基因，对应图2。
表S4：每名患者的线粒体突变情况。本表仅收录至少在两个细胞中检测到突变的位点。WT为野生型（wild type），Undet为测序reads覆盖度低于9X（undetected），Heter为杂合突变（heterozygous mutation），Homo为纯合突变（homozygous）；T与N分别代表肿瘤组织与正常组织。
表S5：OLFM4+SOX9-与OLFM4+SOX9+肿瘤细胞间的差异表达基因，对应图S10A。
表S6：单细胞靶向cDNA桑格（Sanger）测序结果。框内数字为细胞条形码（cell barcode）编号；绿色、橙色与红色分别代表野生型、杂合突变型与纯合突变型，无颜色填充代表未检测到。
表S7：BRAFV600E与BRAF600WT肿瘤细胞间的差异表达基因，对应图S10C-D。
表S8：KRAS野生型（KRASWT）与KRAS突变型（KRASmut）肿瘤细胞间的差异表达基因，对应图S10H-I。
表S9：样本的体细胞突变情况。
表S10：抗体信息与引物序列。