Visceral adipose tissue contributes to improved insulin resistance after DJB via growth hormone-adiponectin axis. Visceral adipose tissue contributes to improved insulin resistance after DJB via growth hormone-adiponectin axis

To explore the potential mechanism of duodeno-jejunal bypass (DJB) in improving insulin resistance in diabetic rats, we suggest that adipose tissue is involved in the regulation. To investigate genetic changes in adipose tissue after duodeno-jejunal bypass, we performed RNA-seq analysis to establish the relationship between growth hormone, adiponectin, and insulin sensitivity by measuring changes in growth hormone pathways, including downstream JAK2 and MAPK pathways, and investigated non-PPAR signaling pathways. This study deepens our understanding of the complex mechanism by which DJB affects insulin resistance. This meticulous exploration fills an important gap in the literature and may pave the way for more targeted and effective diabetes management interventions. Overall design: In order to study the effect of duodenal-jejunal bypass on adipocytes, we took samples from mesentery and groin of T2DM rats and post-DJB rats, representing visceral fat and subcutaneous fat, respectively, and analyzed the difference of gene expression in adipose tissues of different parts by RNA-seq, analyzed the changes of fat function after duodenal-jejunalbypass, and explored the potential mechanism of fat cell function changes.

为探究十二指肠-空肠转流术（duodeno-jejunal bypass, DJB）改善糖尿病大鼠胰岛素抵抗的潜在机制，本研究提出脂肪组织参与了该调控过程。为探究十二指肠-空肠转流术后脂肪组织的基因表达变化，本研究通过RNA测序（RNA-seq）分析生长激素通路（包括下游JAK2与MAPK通路）的表达变化，明确生长激素、脂联素与胰岛素敏感性之间的关联，并对非PPAR信号通路进行了研究。本研究加深了对DJB影响胰岛素抵抗的复杂机制的认知。此次细致的探索填补了该领域文献中的一处重要空白，可为更具针对性与有效性的糖尿病管理干预手段提供研究基础。整体实验设计：为探究十二指肠-空肠转流术对脂肪细胞的影响，本研究分别从2型糖尿病（Type 2 Diabetes Mellitus, T2DM）大鼠与十二指肠-空肠转流术后大鼠的肠系膜与腹股沟处采集样本，前者对应内脏脂肪，后者对应皮下脂肪；通过RNA测序（RNA-seq）分析不同部位脂肪组织的基因表达差异，解析十二指肠-空肠转流术后脂肪功能的变化情况，并探究脂肪细胞功能改变的潜在机制。