Gene expression profiles of EZH2 knockdown

Enhancer of Zeste Homolog 2 (EZH2) is a histone methyltransferase that catalyzes the trimethylation of histone H3 lysine 27 (H3K27me3). EZH2 expression is significantly upregulated in MPNST. In our study, we investigated the function of EZH2 and the molecular mechanisms that are regulated by EZH2 in MPNST pathogenesis. Our findings enhance the knowledge of EZH2’s function and biology, and have the potential to provide a novel therapeutic approach for MPNST patients in the clinic. An EZH2 knockdown experiment was carried out in MPNST cells. There are one control and one siEZH2 samples for two cell lines (724 and 462) and two repeats for each cell line, so in total there are 8 samples. Two group comparison

Zeste同源增强子2（Enhancer of Zeste Homolog 2，EZH2）是一种组蛋白甲基转移酶，可催化组蛋白H3赖氨酸27位点的三甲基化修饰（H3K27me3）。在恶性周围神经鞘膜瘤（MPNST）中，EZH2的表达水平显著上调。本研究探讨了EZH2的功能，及其在MPNST发病过程中调控的分子机制。本研究结果深化了对EZH2功能与生物学特性的认知，有望为临床MPNST患者提供全新的治疗策略。研究团队在MPNST细胞中开展了EZH2敲低实验：针对724和462两种细胞系，分别设置1个对照样本与1个靶向EZH2的小干扰RNA（siEZH2）样本，且每种细胞系设置2次生物学重复，最终共计8个样本。本研究将进行两组样本的比较分析。