HP1c (MO 462).2-4 hr OR embryo

modENCODE_submission_3022 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 2-4hr OR; Genotype: wild type; NUMBER OF REPLICATES: 5; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody HP1c (MO 462) (target is HP1c); Developmental Stage Embryo 2-4hr OR

modENCODE_submission_3022 本提交源自Gary Karpen负责的modENCODE项目。modENCODE项目完整列表请参阅http://www.genome.gov/26524648。
项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）基因组中的125种染色体蛋白。所研究的蛋白参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。本研究将采用染色质免疫共沉淀（Chromatin ImmunoPrecipitation，ChIP）结合基因组平铺芯片技术（即ChIP-chip）。实验将首先利用三种细胞系与两个胚胎阶段的染色质开展蛋白定位分析，后续将针对部分蛋白将分析范围拓展至另外四种动物组织或发育阶段。
数据使用条款与规范请参阅：http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
实验类型：ChIP-chip（染色质免疫共沉淀-基因组平铺芯片）。
生物样本来源：品系：Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；发育阶段：胚胎2-4小时 OR；基因型：野生型。
重复次数：5次。
实验因素：品系Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；抗体：HP1c (MO 462)（靶标为HP1c）；发育阶段：胚胎2-4小时 OR