DNA methylation profiling of human THP-1 monocytes stimulated with arachidonic acid (AA) or oleic acid (OA) and controls. Homo sapiens

High-density array-based DNA methylation profiling of human THP-1 monocytes stimulated for 24 h with 1, 10 or 100uM AA, or the same concentrations of OA, and unstimulated controls. The Infinium HumanMethylation450 BeadChip was used to obtain DNA methylation profiles across approximately 485,000 CpGs. Overall design: Bisulphite converted DNA was hybridised to the Illumina Infinium 450k Human Methylation Beadchip

本数据集针对经1、10或100μM花生四烯酸（Arachidonic Acid, AA）、相同浓度油酸（Oleic Acid, OA）刺激24小时的人THP-1单核细胞，以及未刺激对照组，开展基于高密度阵列的DNA甲基化谱分析。研究采用Infinium人类甲基化450K微珠芯片（Infinium HumanMethylation450 BeadChip），在约485000个CpG位点范围内获取DNA甲基化图谱。整体实验设计为：将经亚硫酸氢盐转化的DNA与Illumina Infinium 450k人类甲基化微珠芯片进行杂交。